علوم زیستی دریا
Seyed Mahmoud Rabiei; Sakineh Yeganeh; Mina Esmaeili Kharyeki
Abstract
In the present study, the antioxidant properties of protein hydrolysate produced from Caspian mullet by-products at different hydrolysis times were investigated. For this purpose, 20 pieces of Mullet fish with average weight of 300±50 g were prepared, and their by-products including head, fins ...
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In the present study, the antioxidant properties of protein hydrolysate produced from Caspian mullet by-products at different hydrolysis times were investigated. For this purpose, 20 pieces of Mullet fish with average weight of 300±50 g were prepared, and their by-products including head, fins and viscera were used for hydrolysis by alcalase enzyme 2.4 L (concentration 1.5%, temperature 55 ° C and pH 8) at different hydrolysis times including 30, 60, 120 and 180 minutes. Results showed that protein and ash content of protein hydrolysate powder were significantly increased and fat content significantly decreased compared to the raw samples (p <0.05). The soluble protein and the degree of hydrolysis of Mullet by-products increased with increasing the hydrolysis time up to 120 min (p>0.05). Free radical scavenging (DPPH) assay showed that increased with increasing the protein concentration up to 120 min. (p<0.05). The lowest IC50 value of DPPH scavenging activity was obtained at 120 and 180 min (1.04±0.06 and 1.01±0.01 mg/ml, respectively) (p<0.05). With increasing protein concentration and time of hydrolysis, the reduction ferric ion power increased significantly at all times and protein concentrations (p <0.05). Moreover, ABTS increased with increasing hydrolysis time up to 120 min (p <0.05). In general, due to the proper performance of the Mullet by-products protein hydrolysate on DPPH and ABTS free radicals scavenging and the ferric ion reduction, it can be stated that, this product can be used as an antioxidant compound.
علوم زیستی دریا
Milad Adel; Reza Safari; Sekineh Yeganeh; Sharareh Ahmadvand
Abstract
This study was conducted to evaluate the effects of different levels of prebiotic GroBiotic®-A on growth factors, survival rate, body composition and intestinal microbiota of cultured juvenile beluga (Hous huso) in the sturgeon culture center (Samandak, Sari). Four groups of beluga sturgeon with ...
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This study was conducted to evaluate the effects of different levels of prebiotic GroBiotic®-A on growth factors, survival rate, body composition and intestinal microbiota of cultured juvenile beluga (Hous huso) in the sturgeon culture center (Samandak, Sari). Four groups of beluga sturgeon with mean weight of 40.82± 5.8 g were raised for 56 days in fiber glass tanks (20 fish to each tank) and feeding with different levels of GroBiotic®-A with concentrations of % 0, % 0.5, %1.0 and % 2.0 (Three replicates were used for each concentration). At the end of the trial, growth factors (final weight, weight gain, SGR, CF feed conversion ratio (FCR), body composition and intestinal microbiota (total viable bacteria and Lactobacillus spp. levels) were determined and compared with control group. Our results confirmed that juveniles fed on diet supplemented with 2% GroBiotic®-A had significantly higher growth factors and , survival rate compared to control and 0.5% treatment (P0.05). Total viable bacteria and Lactobacillus spp. count were increased during this study and these parameters significantly higher in 2% treatment compared to other treatments (P